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This is concurrent with the earlier studies where the ethyl acetate extract of Tinosporacordifolia showed high antiproliferative activities against lung carcinoma cells, murine lewis lung carcinoma (LLC) cells, A549 human lung adenocarcinoma cells in the DNA fragmentation assay (Rudeewan Tungpradit et al., 2010).. Qualitative analysis of Hexane, Ethyl Acetate, Methanol Extract of Tinosporacordifolia leaves PHYTOCHEMICAL TEST Carbohydrates Tannins Flavonoids Quinones Terpenoids Coumarins Steroids phytosteroids HEXANE + + + P+ ETHYL ACETATE + + + S+ METHANOL + + + + + + - + indicates Present, - indicates absent, S indicates Steroids, Pindicates Phytosteroids Table 2.. Antibacterial activity of Tinosporacordifolialeaf extracts by agar diffusion assay Zone of inhibition (mm) Control Methanol (Ciproflaxcin) 8±1.0 30±0.01 Pathogens Hexane Aeromonas hydrophila Pseudomonas aeruginosa Staphylococcus aureus 14±0.5 Ethyl acetate 16±1.1 Nil 18±0.3 24±0.8 20±0.01 Nil 20±0.5 30±0.5 50±0.01 Values are mean of inhibition zone (mm) ± S.D of triplicates.. Determination of Minimal Inhibitory Concentration (MIC) of ethyl acetate and methanolic extracts of Tinosporacordifolia leaves against pathogenic bacteria Organisms Aeromonashydrophila Pseudomonas aeruginosa Staphylococcus aureus Leaf Extracts (mg/ml) Ethyl acetate Methanol 79.67 62 51.03 67.38 64.53 64.55 Fig.. Lane 1: 1kb Ladder, Lane 2: DNA from HT29 cells treated with 50µg Ethyl acetate extract, Lane 3: DNA from HT29 cells treated with 100µg Ethyl acetate extract, Lane 4: DNA from HT29 cells treated with 150µg Ethyl acetate extract Conclusion Throughout history plants have been used by human beings for medicinal purposes and even in modern times have formed the basis of many pharmaceuticals in use..
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