Measurement Process Flow Chart

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Measurement Process Flow Chart (EXAMPLE: Nanotoxicity Assay) Seed Cells Add Nanoparticles Incubate 24 h Remove Supernatant Treat with MTS Reagents Measure Abs490 Measurement Process Flow Chart (TEMPLATE) Measurement Controls (TEMPLATE) Variables/Factors EXAMPLE: Nanotoxicity assay: Variable: Cell toxicity response Why an Issue Cell response varies due to many factors (passage, incorrect seeding, variability in medium, contamination, etc.) Control Experiment /Approach Add cadmium sulfate to cells to establish a reproducible “toxic” response in the assay Reference Material Cadmium sulfate Ishikawa Diagram (EXAMPLE: Nanotoxicity Assay) Rösslein M, Elliott JT, Salit M, Petersen EJ, Hirsch C, Krug HF, Wick P..

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